Describe the process of PCR, including what is needed.

Chemical components needed: a DNA template, DNA polymerase enzyme, primers, nucleotides and reaction buffer.

Steps in PCR:

  1. Denaturation 96 degrees, to seperate DNA strands

  2. Annealing 55-65 degrees, cool so that primers can bind to their complementary sequences

  3. Extension 72 degrees, raise temperature so that polymerase can work to extend primers making new DNA

Answered by Kavi S. Biology tutor

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