Describe the process of PCR, including what is needed.

Chemical components needed: a DNA template, DNA polymerase enzyme, primers, nucleotides and reaction buffer.

Steps in PCR:

  1. Denaturation 96 degrees, to seperate DNA strands

  2. Annealing 55-65 degrees, cool so that primers can bind to their complementary sequences

  3. Extension 72 degrees, raise temperature so that polymerase can work to extend primers making new DNA

Answered by Kavi S. Biology tutor

2132 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

What is the structure of DNA?


How does an atheroma increase the risk of death?


How is gas exchange in a human facilitated?


Explain 3 ways in which red blood cells are adapted to perform their function efficiently


We're here to help

contact us iconContact usWhatsapp logoMessage us on Whatsapptelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2024

Terms & Conditions|Privacy Policy
Cookie Preferences