Describe the process of PCR, including what is needed.
Chemical components needed: a DNA template, DNA polymerase enzyme, primers, nucleotides and reaction buffer.
Steps in PCR:
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Denaturation 96 degrees, to seperate DNA strands
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Annealing 55-65 degrees, cool so that primers can bind to their complementary sequences
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Extension 72 degrees, raise temperature so that polymerase can work to extend primers making new DNA
KS
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