During the S phase of interphase, the DNA is replicated in a semi-conservative manner. The parent strands are seperated and each one is then used to synthesise a new daughter strand through the addition of complementary nucleotides which follow base-pairing rules. During prophase of mitosis, the nuclear material (chromatin) condenses to form chromosomes, which exist as two genetically identical sister chromatids bound by a centromere. Chromosomes line up along the equator of the cell during metaphase, and sister chromatids are subsequently seperated and pulled to opposite ends of the cell during anaphase. The cell is cleaved in two following telophase, forming two genetically identical daughter cells.