What sort of inhibitor in an enzyme catalysed reaction is NOT affected by substrate concentration?

Non competitive inhibitors bind to a site other than the active site, called the allosteric site. As a result of this, the 3D tertiary structure of the enzyme is damaged and the enzyme is no longer capable of catalysing a reaction (remember: the tertiary structure damage means that the active site is also damaged and therefore, substrate-enzyme complexes can no longer be formed). Since these non competitive inhibitors do not compete with substrate molectules, non competitive inhibitors are not affected by substrate concentration (remember: even if you increase substrate concentration, the active sites on enzymes have been damaged and therefore, substrate can not attach to form complexes, the enzymes itself are damaged). 

Answered by Aishwarya S. Biology tutor

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