The 2 DNA strands are split apart by DNA Helicase by breaking the hydrogen bonds between complementary base pairs, with the sense strand being used as a template. Free RNA nucleotides in the cytoplasm attach to the template strand by complementary base pairing. RNA polymerase joins RNA nucleotides together to form a polynucleotide. The polynucleotide detaches- this is now pre-mRNA in eukaryotic cells and mRNA in prokaryotic cells. Once the enzymes have passed the H bonds between complementary base pairs reform and the DNA coils back into a double helix. Once a stop codon is reaches, the process is completed