There are a lot of themes in Frankenstein, and I think which ones are major and which aren't is something we'll be debating. In terms of identifying them, though, we can list them or draw them up on a spider diagram. Scientific advancement, for sure, might be the sensible place to start: Shelley might be suggesting that science can go too far, creating unnatural and dangerous things. We might draw a few themes off the back of that: the power of nature, perhaps, or the role of God and religion in creating life. On the other hand, we might decide that science isn't the truly important theme-- maybe it doesn't matter how the Creature was created, but how Frankenstein deals with him. In that case, we might consider parenthood as a theme, and the idea of nature vs nurture. The important thing is to bear in mind how these themes can lead to various opposing arguments and, as our discussion goes on, we might consider which side we're each on, and start thinking about which textual quotations contain the themes supporting each of our arguments.