Glycolysis is a metabolic process that takes place in the cytoplasm of a cell and occurs in both aerobic and anaerobic respiration pathways. One six-carbon compound is converted into two three-day on molecules, known as pyruvate.1. Phosphorylation: The six-carbon compound (usually glucose) is phosphorylated to form a hexose bisphosphate (6C), consuming two molecules of ATP. This hexose bisphosphate is less stable and more reactive, hence it is trapped inside the cell.2. Lysis: The hexose bisphosphate is split into two triose phosphate (3C) molecules.3. Oxidation: The two triose phosphates are oxidised. A Hydrogen atom is removed from each molecule and transferred to two NAD+ molecules, forming their reduced form, NADH. These function as electron carriers (essential for later steps in the aerobic respiration pathway).4. Substrate level phosphorylation: The energy released from these carbon compounds is used to drive the phosphorylation of four ADP (adenosine diphosphate) molecules into four ATP (adenosine triphosphate) molecules. The remaining 3C compounds are two pyruvate molecules.
(If glycolysis occurs under aerobic conditions, pyruvate will be utilised in the next step of respiration, the Link Reaction)
Overall, per molecule of glucose, glycolysis produces:2x pyruvate2x NADH2x ATP (net total: 2x ATP consumed Vs 4x ATP formed)