Mitosis takes place after interphase in which the DNA in the nucleus replicates in preparation for the cell to become two daughter cells. Prophase is the first stage wherein the duplicated DNA chromosomes condense, the nuclear envelope surrounding the DNA begins to break down and the mitotic spindle, made up of microtubules, forms at polar ends of the cell. Metaphase come next when the chromosomes line up down the centre of the cell and attach to the spindle microtubules by kintechores at the centromere of the chromosomes. During Anaphase the sister chromatids separate and are pulled to polar ends of the cell by the spindle. Finally in Telophase the chromosomes begin to decondense as a nuclear envelope forms around them at each end of the cell and the mitotic spindle breaks down. The cell is now ready to go through cytokinesis, thereby producing two separate daughter cells.