what factors determine enzyme activity?

several factors play a role in the rate of an enzyme catalysed reaction. substrate concentration: The amount of substrate in the solution. The higher the concentration of substrate the more frequently they will collide with enzymes, increasing the frequency of successful collisions to form an enzyme-substrate complex and increase rate of reaction.Enzyme concentration: The amount of enzymes in the solution. This factor works in the same way as substrate concentration.Temperature: A higher temperature means that the substrate has a larger amount of energy, moving faster. This causes more particles to have the required activation energy for the reaction and increases frequency of collisions. however, too high a temperature can break the hydrogen bonds in the enzymes, denaturing the, and preventing the reaction.pH: enzymes work within pH ranges for reactions, outside of these ranges they can become denatured and no longer form an enzyme-substrate complex, reducing rate of reaction.