Under 0 degree celcius, ice crystals will begin to form in the cell membrane of the enzyme. If the temperature increases and the enzyme thaws, the pierced cell membrane will cause leakage and prevent the enzyme from working effectively. Between 10 and 45 degrees celcius, the enzyme works at optimum temperature and it’s function is not impaired. Any enzyme- controlled reaction will work faster as the heat increases. This is due to the molecules of the enzyme haveing more energy and this vibrating more rapidly. At temperatures over 45 degrees celcius the enzyme will begin to die or denature, causing the enzyme to be less effective in reactions.