An increase in temperature increases kinetic energy of particles, so collision frequency increases and more enzyme-substrate complexes are formed, therefore rate of reaction increases. The rate of reaction increases until the optimum temperature which is the temperature at which the highest rate of reaction can be achieved. However, if the temperature increases beyond this, the enzyme will denature. The bonds in the enzyme break down and the shape of the enzyme will change, including the shape of the active site. Once the active sight changes shape the substrate can no longer fit so no enzyme substrate complexes can be formed. This is a permanent change to the enzyme, so as temperature increases further, more enzymes will denature so fewer enzyme-substrate complexes can be formed. Once all enzymes have been denatured the rate of reaction will decrease to zero.