Name 3 things that can affect the activity of enzymes

1) Temperature: increasing the temperature increases the rate of reaction, however this can only happen to a certain point as beyond each enzyme's optimum temperature they get denatured (active site changes shape).2) pH: each enzyme has its own optimum pH where it works best. Too low or too high pH can change the enzymes active site shape, meaning it won't work as well or is denatured.3) Substrate concentration: increasing the amount of substrate increases the number of collisions with the enzymes, therefore increases the rate of reaction. However, this can only happen to a certain point as the enzymes will become saturated, and no more substrates can fit at any one time even though there are plenty available.