Acetylcholine is a neurotransmitter used by cholergenic neurones.Neurotransmitters are chemicals used as a signalling molecule across a synapse.Acetylcholine will be released from the presynaptic bulb from vesicles, pass across the synaptic cleft and eventually bind with receptors on the post synaptic membrane.Any acetylcholine left over will be recycled by the enzyme acetylcholinesterase, an enzyme in the cleft.