Describe the Benedict's test for reducing sugars and how this can be modified to test for non-reducing sugars
Take 1-2cm3 of the sample in a test tubeAdd a few drops of Benedict's reagent to the test tube Heat the test tube in a water bath Leave for 5 minutes Observe the colour of the precipitate formed
no precipitate formed = negative for reducing sugarsprecipitate formed = positive for reducing sugars(green -> yellow -> orange -> brick red) increasing concentration of reducing sugars present
modify for non reducing sugarstake a new sample in a clean test tube add dilute HClwarm (at a lower temperature) in a water bath remove from the water bath and add NaHCO3add Benedict's solution repeat steps 3-5 with this sample
