What techniques were employed in the 'Calvin Experiment' to elucidate the carboxylation of RuBP (IB-HL 8.3)?

Calvin carried out ‘feeding experiments’ where radioactive labelled carbon was fed to plants. 14CO2 is taken up the cells and is then fixed into photosynthesis. 14CO2 was induced into the plants and monitored. Samples of photosynthesising cells taken at frequent intervals after feeding contained a sequence of radioactively labelled intermediates and products of the photosynthetic pathway. They were then isolated by chromatography. Chlorella was used, instead of mesophyll tissue, because they are easy to sample and have identical photosynthesis pathways 

Answered by Adam K. Biology tutor

2749 Views

See similar Biology IB tutors

Related Biology IB answers

All answers ▸

What are the differences between DNA and RNA?


What are the properties of water?


What is meant by the redundancy of the genetic code?


Name the key differences between Eukaryotic and Prokaryotic cells?


We're here to help

contact us iconContact usWhatsapp logoMessage us on Whatsapptelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2025

Terms & Conditions|Privacy Policy
Cookie Preferences