How does column chromatography work? (A-Level Chemistry)

We use column chromatography to purify an organic product. We begin with a glass column filled with a slurry of absorbent material. This could be aluminium oxide coated with water.We run a solvent through this column.As in all chromatography, we must have a stationary phase and a mobile phase. The stationary phase is the absorbent material. The mobile phase is the solvent.As our organic mixture (which we are purifying) drains through the column, the different phases of the mixture separate. This works because different phases will have different adsorptions (which can be thought of as attraction) to the stationary phase, and different solubility in the mobile phase. This will affect the speed at which the phases move through the column, so different phases will leave at different times, and the material is separated.

NM
Answered by Noa M. Chemistry tutor

2494 Views

See similar Chemistry GCSE tutors

Related Chemistry GCSE answers

All answers ▸

Explain four ways of increasing the rate of a reaction


Describe what happens when 2 atoms of potassium react with 1 atom of sulphur? Give the answer in terms of electron transfer.


In aqueous solution, sulphuric acid dissociates into ions in 2 stages. The pKa for the first dissociation is -3. Calculate the pH of a 0.025 mol dm-3 solution of sulphuric acid using the pKa value of the 1st dissociation.


Will a solid ionic compound conduct electricity ?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

MyTutor is part of the IXL family of brands:

© 2026 by IXL Learning