DNA replication is used to copy DNA. This process is semi-conservative, meaning the new double helix contains both a parent and a new strand. The process begins during S phase, where helicase unwinds the DNA helix in a 5' to 3' direction. This breaks the hydrogen bonds between bases. On the leading strand, RNA primase adds primers complementary to the bases on the parent strand. DNA polymerase III then adds dNTPs to complete the strand, which lose two phosphate groups to ensure a continuous formation of the leading strand strand. The primer is removed by DNA polymerase I. On the lagging strand, RNA primase again adds primers, followed by DNA polymerase III adding dNTPs in a 5' to 3' direction. However, this movement is away from the replication fork and does not allow for continuous assembly. Okazaki fragments are therefore left when DNA polymerase I removes the primer. These fragments are joined together by DNA ligase, which makes sugar/phosphate bonds allowing a full strand to be synthesised.