Explain how amino acids could be identified using gel electrophoresis

The sample of amino acids is placed in the center of a polyacrylamide gel, across which a voltage is applied. Depending on the pH of the buffer, the amino acids will move at different rates towards either the positive or negative electrodes . Amino acids will stop moving once they have reached their isoelectric point- the pH at which amino acids have no overall charge. When separation is complete, the amino acids are sprayed with ninhydrin and can be identified by comparing the distance traveled with known standards. 

EH
Answered by Eleanor H. Biology tutor

12966 Views

See similar Biology IB tutors

Related Biology IB answers

All answers ▸

Explain the process of muscle contraction


Describe the process of spermatogenesis leading to the production of four sperm cells in a human male.


Explain how proteins act as enzymes, including control by feedback inhibition in allosteric enzymes [9 marks]


Where in the nephron does osmoregulation occur?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

MyTutor is part of the IXL family of brands:

© 2026 by IXL Learning