Explain how amino acids could be identified using gel electrophoresis

The sample of amino acids is placed in the center of a polyacrylamide gel, across which a voltage is applied. Depending on the pH of the buffer, the amino acids will move at different rates towards either the positive or negative electrodes . Amino acids will stop moving once they have reached their isoelectric point- the pH at which amino acids have no overall charge. When separation is complete, the amino acids are sprayed with ninhydrin and can be identified by comparing the distance traveled with known standards. 

EH
Answered by Eleanor H. Biology tutor

13189 Views

See similar Biology IB tutors

Related Biology IB answers

All answers ▸

Explain the role of Calcium in muscle contraction


What are the differences between normal cells and cancer cells?


Distinguish between active and passive immunity


Outline three outcomes of the sequencing of the complete human genome.


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

MyTutor is part of the IXL family of brands:

© 2026 by IXL Learning