How does DNA replication work?

DNA replication is called semi-conservative, because each original strand is used to create a new strand. In order to do this, first we need to separate the two strands: they are unwound and unzipped by the enzymes DNA gyrase and DNA helicase, to leave two separate strands with the nitrogenous bases exposed. Next, free activated nucleotides are "matched" to the complementary exposed bases on each strand (A to T, C to G). The enzyme responsible for this is called DNA polymerase. Hydrogen bonds form between the complementary bases, and DNA polymerase ensures a sugar phosphate backbone forms on the new strand. However, DNA polymerase works in only one direction, and we know that DNA strands run antiparallel (one 3' to 5' and one 5' to 3'). On the strand where DNA polymerase is not able to smoothly move down the strand (because it keeps hitting un-separated DNA), new DNA forms in small chunks called Okazaki fragments, which are joined by a DNA ligase enzyme.

ET
Answered by Ellie T. Biology tutor

3116 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

Describe the structure and bonding that contributes to a functional protein.


Describe how the alveoli are adapted for gas exchange


How would you test for the presence of a non-reducing sugar?


Describe and explain the process by which proteins are created from DNA


We're here to help

contact us iconContact usWhatsapp logoMessage us on Whatsapptelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2025

Terms & Conditions|Privacy Policy
Cookie Preferences