'Ser' and 'estar' refer to the verb 'to be'. However, in Spanish the verb 'to be' can be used in different ways depending on the context. For example 'estar' refers to a an emotional state that can vary with time eg. 'estoy enamorado' (I am in love) or 'estoy de buen humor' (I am in a good mood) , while 'ser' would be used when you are talking about something permanent that is a characteristic unlikely to change eg. 'Soy de Madrid' (I am from Madrid) or 'Eres muy gracioso' (You are very funny)