Explain how restriction endonucleases are used to form recombinant DNA plasmids.
Endonucleases cut DNA at palindromic sites producing 'sticky ends'. The endonuclease cuts the plasmid DNA and the DNA to be inserted at the same palindromic site. The sticky ends of the plasmid DNA and the insert DNA are complimentary and form hydrogen bonds. DNA ligase sticks the DNA together to form a recombinant DNA plasmid.
TD
Related Biology A Level answers
All answers ▸Describe the process of translation.
John begins to exercise and his heart rate increases - explain how and why his heart rate increases
What is the main function of the Schwann cell?
Define the term 'water potential' and describe the difference between isotonic, hypotonic and hypertonic solutions. Suggest the different effects on cells placed in the different solutions.