What are the steps involved in the mark-release-recapture technique and which factors does it rely upon?

Mark-release-recapture is a technique used to estimate the size of a population of a particular species. The steps involved are:1. Capture and count animals.2. Mark them in a way which is not toxic/harmful to them.3. Release them back into the community.4. Capture a second sample and count them.5. Record the number of marked individuals recaptured.The estimated population size is:(total no. in 1st sample x total no. in 2nd sample) / no.of marked individuals recapturedHowever, this technique only works if the following factors are in place:1. There are few births and deaths in the population.2. The population has a definite boundary; there is no emigration or immigration.3. The proportion of marked:unmarked individuals in the second sample is the same as the proportion in the population as a whole.4. Marked individuals from the first sample distribute themselves evenly amongst the rest of the population and have sufficient time to do so.5. The method of marking is non toxic / does not make them more conspicuous to predation.6. The method of marking is not lost / rubbed off during the investigation.

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